羟乙基与N-琥珀酰羟乙基壳聚糖的制备表征及细胞毒性试验

Preparation,characterization and cytotoxicity of hydroxyethyl chitosan and N-succinyl-hydroxyethyl chitosan

壳聚糖(chitosan,CTS)与氯乙醇反应制备羟乙基壳聚糖(hydroxyethyl chitosan,HECTS),并接合琥珀酸酐合成N-琥珀酰羟乙基壳聚糖(N-succinyl-hydroxyethyl chitosan,Suc-HECTS),考察琥珀酰化度的影响因素确定最适反应条件。用FT-IR对产物进行结构表征,1 H NMR测定脱乙酰度和琥珀酰化度,元素分析法测定羟乙基取代度,并评价了HECTS和Suc-HECTS的细胞毒性。结果表明HECTS与Suc-HECTS均无细胞毒性。在生物医药和药物载体方面的应用提供了一定的理论基础。

A water-soluble chitosan derivative hydroxyethyl chitosan（HECTS） was synthesized by chitosan（CTS） and chlorethanol.HECTS reacted with succinic anhydride and generated N-succinyl-hydroxyethyl chitosan（Suc-HECTS）.The best reaction condition was selected by analyzing the experiment conclusions in different conditions to obtain the supreme binding rate of succinic anhydride.The acquired products were characterized by FT-IR.The deacetylation degree,binding rate of succinic anhydride and substitution degree of hydroxyethyl were measured by 1H NMR and elemental analysis.Cytotoxicity of HECTS and Suc-HECTS were evaluated,and the results showed that two materials both had noncytotoxicity.The experimental conclusions offered a theoretical basis for the application of HECTS and Suc-HECTS as biopharmaceutical material and drug carrier.