独活对阿尔茨海默病模型大鼠免疫损伤干预作用的实验研究

The Experimental Study of the Intervention Effect of Duhuo on Immune Damage of Model rats Suffering From Alzheimer

目的:研究独活颗粒剂对Aβ1–40蛋白所诱发的AD模型大鼠免疫损伤的抑制作用,探讨独活延缓脑老化的作用机制。方法:采用Aβ1–40灌注法建立AD大鼠模型,分别用中药独活颗粒剂、西药吲哚美辛、0.9%的氯化钠溶液灌胃,选择ELISA双抗体夹心方法,检测各组大鼠血清中细胞因子IL-4、TNF-α的表达变化。结果:与假手术组比较,AD模型大鼠血清TNF-α的含量增加(P<0.01),IL-4的含量减少(P<0.01);与模型组比较,独活组大鼠血清TNF-α的含量减少(P<0.01),IL-4的含量增加(P<0.01)。结论:独活颗粒剂对Aβ1–40蛋白所诱发的老年性痴呆病(AD)模型大鼠的炎症反应具有抑制作用;独活颗粒剂通过调节炎症反应而具有明显的延缓脑老化作用。

To investigate the mechanisms of Duhuo to delay cerebral aging through studying inhibitory effect of Duhuo water granula on immune injury in AD model of Wister rats.Method：To use Aβ-amyloid protein injection method of Wister rats and Hippocampus to shape AD model,traditional Chinese medicine Duhuo,Western medicine indomethacin,and saline were administered.ELISA method is used to detect expression of cytokines in serum IL-4 and TNF-α in different groups.Results：The content of IL-4 in AD model Wister rats was lower（P0.01）than that of general Wister rats,whereas the content of TNF-α was higher（P0.01）.Compared with model group,IL-4 secretion in the Chinese medicine group was higher（P0.01）.whereas the content of TNF-α was lower（P0.01）.Conclusion：Duhuo could inhibit the inflammation of central nerves of AD model Wister rats.Duhuo water granula could delay cerebral aging and the mechanism is to regulate immune function so as to prevent and trent immune injury.