糖尿病大鼠肺组织基质金属蛋白酶-2,9的变化及罗格列酮的作用

The changes of matrix metalloproteinase-2,9 in lung tissue in diabetic rats and the role of rosiglitazone in the changes

目的探讨罗格列酮(RGZ)对DM大鼠肺间质纤维化的作用。方法通过高糖、高脂饮食加小剂量链尿佐菌素(STZ)建立DM大鼠模型,随机分为对照组(Con组)、DM组、RGZ组。采用Masson染色观察肺组织胶原含量;免疫组化法观察肺组织基质金属蛋白酶-2,9(MMP-2,9)的变化。结果 DM大鼠肺组织出现了胶原纤维聚集,细胞外基质增加,RGZ大鼠肺组织的胶原聚集明显减轻;DM大鼠肺组织MMP-2,MMP-9的水平明显高于Con组,RGZ组MMP-2,MMP-9的水平显著低于DM组。结论 DM大鼠出现了肺间质纤维化,肺组织MMP-2,MMP-9的水平升高与肺间质纤维化的发生有关,RGZ降低了DM大鼠肺组织MMP-2,MMP-9的含量,减轻了DM大鼠肺间质纤维化的程度。

Objective To investigate the role of rosiglitazone （RGZ） on pulmonary fibrosis in diabetic rats. Methods The experimental diabetic rats were yielded by injecting streptozotocin and feeding with high fat and high glucose food. All rats were randomly assigned to control, DM and RGZ group. The collagen accumulation of lung was examined by masson trichrome staining. The protein expressions of MMP-2, MMP-9 in lung were examined by immunohistochemistry. Results The amount of collagen fiber increased obviously in lung tissue in diabetic rats, and decreased in RGZ treated rats. The level of MMP-2, 9 in lung tissue was higher in diabetic rats than in control group. RGZ decreased the level of MMP-2, 9 in lung tissue. Conclusions Pulmonary fibrosis is present in diabetic rats. The level of MMP-2, MMP-9 in lung tissue of dibtetic rats might be related to lung tissue pathological changes. RGZ may alleviate pulmonary fibrosis by down-regulating the level of MMP-2, MMP-9.