粘细菌AHB103-1代谢产物抗肿瘤活性的初步研究

Elementary Study on the Antitumor Characteristic of the Metabolite Produced by Myxobacteria AHB 103-1

本文对粘细菌AHB103-1的次级代谢产物A组分的抗肿瘤活性和作用机制进行了初步的探讨。采用MTT方法研究了它对HepG2、MDA-MB-231、293T和B16四种肿瘤细胞的作用浓度和作用时间,并与临床上应用的几种抗肿瘤药物对HepG2细胞的作用效果进行了比较;利用荧光显微镜和扫描电镜观察研究了它对HepG2细胞的作用机制。结果表明:A组分的抗癌活性比泰素低,却高于表柔比星、依立替康和奥沙利铂。当样品浓度≥15μg/mL时,对各种肿瘤细胞株的抑制率均达到90%以上。随着样品浓度的降低,对各细胞株的抑制率均表现下降趋势。当样品浓度为30μg/mL时,对四种细胞株作用时间选择24 h即有理想效果。当样品浓度为1.88μg/mL时,对B16、293T和HepG2细胞,作用时间选择48 h较好;而对MDA-MB-231,作用时间选择72 h较好。荧光显微镜和扫描电镜观察的结果分别从形态学上证明了A组分具有引起HepG2细胞凋亡的活性。

The antitumor activity and mechanism of component A produced by myxobacteria AHB103-1 were elementarily discussed in the article,. The MTT method was adapted to study the concentration and the action time of the component A for four tumour cell HepG2, MDA-MB-231,293T and B16, and the effect on HepG2 between it and some clinical drugs was compared,. The fluorescence microscope and electron microscope were utilized to study its action mechanism on HepG2 , The results showed that the metabolite＇ s antitumor activity was lower than paelitaxel, but higher than epirubiein,irinoteean, and oxaliplatin,. More than 90% of inhibition rate for four tumor cell was reached when the sample concentration ≥ 15 μg/mL,. However,the inhibition rate fell with the decreasing of the sample concentration,. On the other hand,the ideal results could be gotten with the handling time for four tumour cell chosen 24 h, when the sample concentration was 30μg/mL,. However, when the sample concentration was 1.88 μg/mL, the suitable handling time should be chosen 48 h for B16,293T and HepG2,but 72 h for MDA-MB-231,. Finally,the observation results by fluorescence microscope and electron microscope respectively proved from morphology the apoptosis of HepG2 induced by the component A.