甲氧补骨脂素对大鼠成骨细胞生物活性影响的实验研究

Effects of 8-Methoxypsoralen on Proliferation and Differentiation of Cultured Osteoblasts in vitro

为探讨甲氧补骨脂素对体外培养新生大鼠颅骨成骨细胞增殖与分化作用的影响,用改良的组织块培养法分离培养新生大鼠颅骨成骨细胞,在成骨细胞体系中以不同浓度加入甲氧补骨脂素,MTT法检测加药后不同时间细胞的增殖情况;用对硝基苯二钠基质动力学法(PNPP)测定细胞内碱性磷酸酶的活性,用改良的Lowry法测蛋白含量;用放射免疫法测定细胞内骨钙素含量。结果显示:与对照组相比,甲氧补骨脂素组在24 h和36 h时促进体外大鼠成骨细胞增殖的作用更明显;在24、48 h和72 h时均能提高成骨细胞碱性磷酸酶活性(ALP)和骨钙素(BGP)的分泌。甲氧补骨脂素对体外培养的大鼠成骨细胞的增殖与分化均有明显的促进作用。

To study the effects of 8-methoxypsoralen on proliferation and differentiation of cultured osteoblasts in vitro, MTT, PNPP and RIA were used separately to observe the proliferation, the activity of ALP and the content of osteocalcin of osteoblasts cultured in vitro with 8-methoxypsoralen of different concentration in it for different incubation periods. Protein was measured by Lowry. The effects of promoting proliferation of rat osteoblasts cells in vitro after 24 h and 36 h were more marked by the groups of 8-methoxypsoralen compared with the control group. The activity of alkaline phosphate （ALP） and secretion of osteocalcin （BGP） were stimulated by 8-methoxypsoralen after 24,48 h and 72 h incubation. It was demonstrated that 8-methoxypsoralen has the effects on stimulating the proliferation and differentiation of cultured osteoblasts in vitro.