鸡冠花子的化学成分研究

Chemical constituents of celosia cristatae semen

目的:分离鉴定鸡冠花子的化学成分。方法:采用硅胶柱色谱、Sephadex-20柱色谱、重结晶及制备高效液相色谱等技术提取分离鸡冠花子的化学成分,用UV、IR、EI-MS、ES-MS、1H-NMR、13 C-NMR、HMQC及HMBC等光谱方法鉴定化合物结构。结果:从鸡冠花子乙醇提取物的石油醚部位分离得到3个化合物,分别鉴定为棕榈酸(1)、对羟基苯甲醛(2)、齐墩果酸(3);从二氯甲烷部位分离得到6个化合物,分别鉴定为stigmast-5-en-3-ol,oleate(4)、4-羟基-3-甲氧基苯甲酸(5)、胡萝卜苷(6)、芹菜素(7)、木樨草素(8)、鸡冠花苷(9)。结论:化合物4～6首次从鸡冠花子中分离得到,化合物9是新化合物。

Objective： To isolate and indentify the chemical constituents of celosia cristatae semen. Methods： Chromalography on silica gel column, Sephadex LH-20 columm, recrysiallization and preparative HPLC technique were used to isolate and purify the compounds. Spectroscopy methods including UV, IR, El-MS, ES-MS, ^1H-NMR,^13C-NMR, HMQC and HMBC were used to elucidate the structures of compolimts. Results： Nine compounds were obtaiined and identified： palmitic acid （1） , 4-hydrobenzal dehyde （2） , oleanolic acid （3） , stigmast- 5-en 3-ol,oleate （4）, 4-hydro-3-methlbenzal acid （5）, daucosterol （6）, apigenin （7）, luteolin （8）, and semenostde A （9）. Conclusions： Compound 4 -6 are isolated from celosia cristatae semen for the first time. Compound 9 is a new compound.