黄芪多糖对早期糖尿病肾病大鼠足细胞nephrin和podocin表达的影响

Effect of astragalus polysaccharin on expression of nephrin and podocin in podocytes of early diabetic nephropathy rats

目的:观察黄芪多糖(APS)对糖尿病肾病(DN)大鼠足细胞裂孔隔膜蛋白分子(nephrin和podo-cin)表达的影响,探讨其防治DN的作用机制。方法:24只健康雄性SD大鼠,随机抽取8只为正常对照组,其余大鼠链脲佐菌素(STZ)腹腔注射复制糖尿病模型,1周后经血糖浓度测量确定大鼠糖尿病建模成功,将造模大鼠分为STZ组(8只)和STZ+APS组(8只),各组分别干预8周。于干预后第2、5、8周检测大鼠血糖浓度;第8周末称量大鼠体重,计算肾指数,观察肾脏病理改变;检测24 h尿蛋白总量t和血尿素氮、血肌酐;蛋白免疫印迹法检测大鼠肾组织nephrin和podocin的表达水平。结果:应用APS干预后,大鼠血糖、肾系数、24 h尿蛋白总量、血尿素氮和血肌酐明显降低(P<0.05),体重增加(P<0.05);病理改变减轻;nephrin和podocin蛋白表达增加(P<0.05)。结论:APS对DN肾脏的保护作用可能与维持足细胞nephrin和podocin的表达有关。

AIM： To observe the effects of astragalus polysaccharin（APS） on the expression of nephrin and podocin in podocytes of diabetic nephropathy（DN） rats.METHODS： The rat model of diabetes was induced by intraper-itoneal injection of streptozotocin（STZ）.The diabetic rats were randomly divided into 2 groups by the treatment without or with APS： STZ group（n = 8） and STZ ＋ APS group（n = 8）.In addition,8 non-treated rats served as control.All the rats were treated with APS or normal saline orally by gavage for 8 weeks.The concentration of blood glucose was monitored on week 2,5 and 8 after treatment.Eight weeks later,the body weight and renal index were measured.Total urine proteinin 24 h,blood urea nitrogen（BUN） and serum creatinine（SCr） were detected by biochemical methods.The pathological changes of the kidneys were also observed under light microscope.The protein levels of nephrin and podocin in the kidney tissues were also determined by Western blotting.RESULTS： After APS intervention,the levels of renal index,blood glu-cose concentration,24-hour total urine protein,BUN and SCr were significantly lower and body weight was higher thanthose in STZ group（P 0.05）.The renal pathological status in APS group was significantly improved and the expression levels of nephrin and podocin also markedly increased（P 0.05）.CONCLUSION： APS might protect kidney against STZ-induced injury via increasing the expression of nephrin and podocin in podocytes.