摘要
目的:研究榛蘑多糖对四氯化碳所致小鼠急性肝损伤的保护作用。方法:将50只小鼠按体重分为5组;分别为正常组、四氯化碳模型组、阳性对照组(联苯双酯200mg/kg/d)、榛蘑多糖大小剂量组(200、100mg/kg/d),每组10只。灌胃7d后,腹腔注射0.1%四氯化碳,24h后取血测定血清丙氨酸氨基转移酶(ALT)和天门冬氨酸氨基转移酶(AST)活性,取肝测定肝脏丙二醛(MDA)含量以及谷胱甘肽过氧化物酶(GSH-Px)活性和还原型谷胱甘肽(GSH)的含量。结果:榛蘑多糖可显著降低四氯化碳所致急性肝损伤小鼠血清ALT和AST活性(P<0.01或P<0.05),也能明显降低四氯化碳所致急性肝损伤小鼠肝脏MDA含量(P<0.05),升高GSH-Px活性(P<0.01或P<0.05)和GSH的含量(P<0.05)。结论:榛蘑多糖对四氯化碳所致小鼠急性肝损伤具有一定的保护作用。
Objective：To observe the protective effects of Armillariella mellea polysaccharide（AMP） on acute liver injury of mice induced by carbon tetrachloride（CCl4）.Methods：Fifty mice were divided into five groups according to the weight;control group,carbon tetrachloride group,positive control group,low and high（200 mg/kg/d,100 mg/kg/d）dosage of AMP groups.All animals were sacrificed 24 hours after administered 0.1% carbon tetrachloride intraperitoneally.Then,the serum and liver tissue were examined by different kits.Results：AMP significantly decrease serum ALT and AST activities（P0.01orP0.05）,liver MDA content（P0.05）,and increase liver GSH-Px activity（P0.01orP0.05）,liver GSH content（P0.05） in CCl4 poisoned mice.Conclusions：AMP has protective activity for CCl4-induced liver injury.
出处
《食品科技》
CAS
北大核心
2011年第10期170-172,177,共4页
Food Science and Technology
基金
国家自然科学基金项目(30760150/c1506)
