提取条件对蓝靛果花色苷抑菌作用的影响

Extracting conditions on the bacteriostasis of Lonicera edulis Turcz.anthocyanin

实验采用微生物浊度法,研究不同提取条件下所得蓝靛果花色苷对大肠杆菌和金黄色葡萄球菌的抑制作用。结果表明:不同水提条件下所得的蓝靛果花色苷对大肠杆菌和金黄色葡萄球菌均有抑制作用。通过正交实验得到对大肠杆菌有抑制作用的蓝靛果花色苷的最佳提取条件为:料液比1:25,时间5h,温度65℃;对金黄色葡萄球菌有抑制作用的蓝靛果花色苷的最佳提取条件为:料液比1:20,时间5h,温度55℃。

Using microorganism nephelometry,we studied the Lonicera edulis Turcz anthocyanins inhibition to E.coli and Staphylococcus aureus under different water extracting conditions.Results showed that the anthocyanins extracted by water can inhibit E.coli and Staphylococcus aureus.By the orthogonal test,we obtained the best conditions for E.coli inhibition：materiel-liquid ratio 1：25,time 5 h,temperature 65 ℃,while the best extraction conditions for staphylococcus aureus inhibition were：materiel-liquid ratio 1：20,time 5 h,temperature 55 ℃.