摘要
研究了采用高效液相色谱同时测定果汁饮料中柠檬黄、日落黄、苋菜红、胭脂红、亮蓝、诱惑红等6种合成着色剂的可行性,同时对果汁饮料样品前处理方法进行了探讨。结果表明,采用AQ-C18色谱柱(250mm×4.6mm),以0.02mol/L的乙酸铵溶液和甲醇为流动相,梯度洗脱,柱温25℃,二极管阵列检测器在波长254nm可以同时对果汁饮料中的6种合成着色剂进行检测;整个分离过程在15min内完成;6种合成着色剂的加标回收率分别在96.6%～103.0%之间。
A HPLC method is proposed for the simultaneous separation and determination of six synthetic colours,tartrazine yellow,sunset yellow,amaranth,ponceau 4R,allura red,brilliant blue in fruit juice.The separation was achieved within 15 min by using a AQ-C18 column（250 mm×4.6 mm） with 0.02 mol/L NH4Ac and methanol as mobile phase and column temperature was 25 ℃,lastly detected at 254 nm through a photo-diode array detector.The average recoveries for real samples ranged from 96.6%,103.0%.
出处
《食品科技》
CAS
北大核心
2011年第10期301-304,共4页
Food Science and Technology
