不同地理品系LdMNPV对取食青杨的亚洲型舞毒蛾幼虫的致死与亚致死作用

THE LETHAL EFFECT AND SUBLETHAL EFFECT OF DIFFERENT GEOGRAPHIC STRAINS LDMNPV FOR THE 2^(nd) INSTAR ASIAN GYPSY MOTH LARVAETH

为了更好的利用LdMNPV对亚洲型舞毒蛾幼虫进行生物防治,本文采用食料给毒法测定了不同地理品系LdMNPV对取食青杨的亚洲型舞毒蛾2龄幼虫的致死与亚致死作用。结果表明:LdMNPV-D、LdMNPV-H、LdMN-PV-J 3个地理品系的LC50分别为333、9、1076 OBs/μL,LT50分别为10.5d1、3.5d、8.9d;亚致死剂量的LdMNPV 3个地理品系对亚洲型舞毒蛾雌雄虫的发育历期、雌雄性比及蛹重均有一定影响,主要的表现为:亚致死剂量的LdMNPV-H和LdMNPV-J品系均延长了亚洲型舞毒蛾发育历期,且与对照相比总虫期达到显著水平;LdMNPV-D品系虽然也延长了亚洲型舞毒蛾的发育历期但总虫期未达到显著水平。亚致死剂量的LdMNPV 3个地理品系降低了亚洲型舞毒蛾的雌雄性比。LdMNPV-D品系对雌雄虫蛹重的影响均达到显著水平;而LdMNPV-J品系对雌虫蛹重的影响达到极显著水平;LdMNPV-H品系对雌雄虫的蛹重均没有显著性的影响。

In order to use of the LdMNPV to Asian gypsy moth larvae to the biological control better,the study use the diet to poison method to determine the lethal effect and sublethal effect of different geographic strains LdMNPV,which to the 2nd instar Asian gypsy moth larvae feeding poplar（Populus cathayana）.The results showed： The LC50s of LdMNPV-D,LdMNPV-H,LdMNPV-J were 33,39 and 1076 OBs/μL and the LT50s of the three isolates were 10.5,13.5 and 8.9d.The sublethal dose of three geography LdMNPV strains have a certain influence to the developmental time,the sex ratio and the pupal weight of Asian gypsy moth larvae.The sublethal dose of LdMNPV-H and LdMNPV-J prolonged the developmental time of Asian gypsy moth,and the total insects stage had significant difference with the contrast;LdMNPV-D also prolonged the developmental time of Asian gypsy moth but the total insects stage had no significant difference with the contrast.The sublethal dose of three geography LdMNPV strains reduced the sex ratio of the females than males.LdMNPV-D strains had significant difference with the pupal weight of female and male ones,but LdMNPV-J strains have highly significant difference with the pupal weight of the female ones.The sublethal dose of LdMNPV-H have no significant effects to the the pupal weight of the female and male ones.