前列腺素E_1对大鼠坐骨神经嵌压性损伤修复的影响

Effect of prostaglandin E_1 on repair of sciatic nerve with crushing lesion in rats

目的探讨前列腺素E1(PGE1)对血管内皮生长因子(VEGF)的表达和周围神经嵌压损伤修复的影响。方法将60只SD大鼠随机均分为生理盐水(A)组、PGE1(B)组、正常对照(C)组。制作坐骨神经嵌压性损害的动物模型,采用免疫组化与电镜分别检测嵌压后12 h、72 h及7 d时大鼠背根神经节VEGF阳性神经元与嵌压后第4周大鼠背根神经节的病理变化。结果嵌压72 h后,A、B组背根神经节VEGF阳性神经元数达到峰值,且明显大于C组(P<0.01),B组VEGF阳性神经元数亦较A组增加(P<0.01)。嵌压后第4周,C组未见病理改变,B组电镜下病变严重程度较A组减轻。结论 PGE1可促进周围神经嵌压性损伤的修复,可能与VEGF表达的增加有关。

Objective To investigate the effect of prostaglandin E1（PGE1） on the expression of vascular endothelial growth factor（VEGF） and the repair of peripheral nerve with crushing lesion in rats.Methods Sixty SD rats were randomly and equally divided into three groups of A（treated with normal saline）,B（treated with PGE1） and C（normal control）.The crushing lesion of sciatic nerve model was constructed.The positive neurons of VEGF in dorsal root ganglion after 12 h,72 h and 7 d of crushing lesion and the pathologic changes of dorsal root ganglion on the 4th week were detected by immunohistochemy and electromicroscope,respectively.Results At 72 h after crushing lesion,the positive neurons of VEGF in dorsal root ganglion reached the peak level in groups of A and B,which were greater than those in group C（P〈0.01）.And the positive neurons of VEGF in group B were also greater than those in group A（P〈0.01）.On the 4th week after crushing lesion,there was no obvious pathologic change in group C,while the severity of pathologic outcomes in group B was slighter than that in group A.Conclusion PGE1 can promote the repair of peripheral nerve with crushing lesion,probably via increasing the expression of VEGF.