摘要
目的研究银/四氧化三铁纳米复合材料(Ag/Fe3O4)对人源性脑胶质瘤U251细胞的放射敏感性的影响。方法人源性脑胶质瘤U251细胞分为对照组、Ag/Fe3O4组、放疗组、Ag/Fe3O4复合放疗组。用集落形成实验检测Ag/Fe3O4对U251细胞放疗效果;流式细胞术检测银纳米颗粒(AgNPs)对细胞周期和凋亡率的影响。结果 Ag/Fe3O4能够引起U251细胞G2/M期阻滞,并使细胞放疗后凋亡率显著提高(P<0.05)。结论可利用Ag/Fe3O4纳米颗粒提高脑胶质瘤放射敏感性。
Objective To study the effect of Ag/Fe3O4 nanoparticles on the efficacy of radiosensitivity enhancement of U251 glioma cells.Methods U251 cells were divided into four groups of control,Ag/Fe3O4,radiotherapy and Ag/Fe3O4 combined with radiotherapy.The effect of Ag/Fe3O4 nanoparticles on radiosensitivity of glioma cells was detected by colony-forming assay.The cell cycles and apoptosis after irritation were tested by flow cytometry.Results Ag/Fe3O4 nanoparticles could induce cell cycle in G2/M arrest and increased cells apoptosis rate after radiotherapy（P〈0.05）.Conclusion The Ag/Fe3O4 nanostructures can significantly enhance radiosensitivity of glioma cells.
出处
《江苏医药》
CAS
CSCD
北大核心
2011年第18期2129-2130,F0002,共3页
Jiangsu Medical Journal
关键词
纳米颗粒
放疗
凋亡
Nanoparticles
Radiotherapy
Apoptosis
