儿童急性呼吸道博卡病毒感染的病毒载量与临床特征相关性研究

The correlational study of viral load of human bocavirus and clinical features in children with acute respiratory tract infection

目的 研究急性呼吸道感染患儿人博卡病毒（ human bocavirus，H BoY）病毒载量与临床特征的相关性。方法 对2009年l1月至2010年12月间956例呼吸道感染的患儿及251例健康对照组儿童鼻咽部抽吸物、咽拭子采用PCR法进行HBoV检测，进而对阳性样本进行实时荧光定量PCR法测定博卡病毒DNA载量，并结合患儿的临床检查进行综合分析。结果 实验组与对照组HBoV阳性率存在显著差异，下呼吸道感染病例HBoV的病毒载量水平与上呼吸道感染病例及对照组儿童差异均有统计学意义，上呼吸道感染病例与对照组儿童病毒载量无统计学意义，重症下呼吸道感染患儿与普通下呼吸道感染患儿HBoV的病毒载量无统计学差异，HBoV混合感染与独立感染患儿病毒载量亦无统计学差异。结论 博卡病毒常年均可引起发病，是儿童呼吸道感染的重要病原体之一，但可能不是儿童急性呼吸道感染的唯一因素。HBoV病毒载量并不能独立反映临床疾病感染的严重程度。

Objective To analyze the correlations between clinical features in paediatric patients with acute respiratory tract infection （ARTI） and viral load of human bocavirus. MethodsA prospective study was conducted on 956 children 〈 5 years admitted with an acute respiratory tract infection from November 2009 to December 2010 ,and 251 healthy children conclused as control group in the corresponding period. Human bocavirus was investigated in nasopharyngeal aspirates（NPA） and throat swab by PCR, and viral load was detected by real-time polymerase chain reaction（RT-PCR） in HBoV positive sample. Clinical data were also prospectively recorded. ResultsA significant difference was found in HBoV positive rate between children with ARTI and control group at enrollment. There was a significant difference in HBoV viral load between children with upper respiratory tract infection and lower respiratory tract infection. HBoV viral load did not differ significantly between children with upper respiratory tract infection and control group. Among children with lower respiratory tract infection, no significant difference were detected between common and severe cases in HBoV viral load. HBoV viral load did not differ significantly whether the children were with or without co-infection. ConclusionsHBoV could be detected perennial and considered as a major pathogen associated with acute respiratory tract infection in children. However, HBoV may not be a independent factor in children with ARTI and the HBoV viral load was not associated with the severity of respiratory illness.