肺癌患者EBV感染、LMP1和Bcl-2表达状况的研究

Study on EB virus infection, LMP1 and Bcl-2 expression in lung cancer patients

目的 研究肺癌患者癌组织中EB病毒（Epstein-Barr virus，EBV）感染、EBV潜伏膜蛋白1 （latent membrane protein 1，LMP1）和B淋巴细胞瘤/白血病-2基因（B cell lymphoma/Leukemia-2,Bcl-2）的表达及意义。方法 采用原位杂交法（in situ hybridization，ISH）检测肺癌组织标本中EBV编码的小RNA（ EBER1），免疫组化法（immunohistochemistry，IHC）检测Bcl-2和LMP1的表达，以GD-6多媒体彩色病理图像分析系统进行形态学定量，以图像的平均面积（average area，AA）和积分吸光度（Integral optical density，IA）表示表达量的多少。结果 在108例肺癌癌组织中36例EBV阳性，阳性率为33.3％；LMP1阳性表达7例，阳性率6.5％。EBV阳性肺癌组中Bcl-2表达显著高于EBV阴性组，其AA分别为58014.23±6918.45和38156.22±4096.79，其IA分别为11.00±1.48和8.03±0.78,差异有统计学意义。进一步分析LMPI和Bcl-2表达的关系，可见LMP1可使Bcl-2表达率增加，但定性分析和定量分析差异无统计学意义。结论 EBV感染使Bcl-2表达增加，可能在肺癌的发生发展中有一定作用。在肺癌组织中EBV可能不是通过LMP1来影响Bcl-2的表达，具体机制有待进一步研究。

Objective To investigate the Epstein-Barr virus （EBV） infection, the expression of EBV latent membrane protein 1 （ LMP1 ） and oncogene bcl-2 in lung cancer patients. Methods EBER1 in 108 cases of lung cancer were detected with in situ hybridization. EBV positive and negative lung cancer tissues were analysed for the expression of LMP1 and Bcl-2 by immnohistochemistry. The average area （AA）and integral optical density （IA） of each sample was measured with the digital medical image analyzing system. ResultsIn 108 cases of lung cancer, 36 cases were EBER1 positive and 7 cases were LMP1positive. The expression of Bcl-2 was higher in EBV positive lung cancer tissues than that in EBV negative.The AA value was 58014. 23 ±6918.45 and 38156.22 ± 4096. 79, while the IA value was 11. 00 ± 1.48 and 8.03 ±0. 78 respectively. No statistic difference was fund in the expression of Bcl-2 betwen LMP1 positive and negative lung cancer tisssues. ConclusionEBV infection in lung cancer increased the expression of bcl-2, which may play a role in the occurrence or development of lung cancer. The increased expression of Bcl-2 may not be induced by LMP1. The exact mechanism need further study.