丙型肝炎病毒核心抗原在静脉吸毒者血清中的检测

Detection of hepatitis C core antigen in intravenous drug addictions

目的 调查丙型肝炎病毒核心抗原（HCVcAg）在静脉吸毒者血清中的检出状况。方法 对93例静脉吸毒者血清同时进行HCVcAg、HCV RNA定量、抗HCV IgG、HBsAg检测，分析HCVcAg检出率与HCV RNA定量及合并HBV感染之间的关系。结果 在93例静脉吸毒者中，抗HCV IgG阳性79例,HCV RNA阳性68例,HCVcAg阳性37例。以HCV RNA作比较，HCVcAg检测的特异性和敏感性分别是100％和54％；HCVcAg检出率与HCV RNA载量对数值呈正相关,r=0.958，P=0.010;HCV RNA阳性静脉吸毒者中，HBsAg阳性和HBsAg阴性时HCVcAg的检出率分别为38％和69％，两者比较P＜0.01;2例抗HCV IgG阴性、HCV RNA阳性静脉吸毒者，HCVcAg检测呈阳性。结论 HCVcAg在静脉吸毒者血清中检测其特异性好，而敏感性较差；HCVcAg的检出率与HCV RNA载量的对数呈正相关；在静脉吸毒者，合并HBV感染可能是血清HCVcAg检测的干扰因素，可能是HBV抑制了HCVcAg的表达。

Objective To study the status of detection of hepatitis C core antigen in intravenous drug addictions, and discuss the foreground of the hepatitis C core antigen ELISA test system. Methods HCV core antigen, HCV RNA quantity, anti HCV-IgG, HBsAg were analysis in all the plasma samples taken from 93 cases of intravenous drug users. Results The specialty and sensitivity of HCV core antigen in intravenous drug addictions 100%-54% separately. When HBsAg were positive, the sensitivity of HCV core antigen was 38% , while HBsAg negative, the sensitivity of HCV core antigen was 69% （ P 〈 0. 01 ）.Conclusion The detections of HCV core antigen showed high specialty but low sensitivity in intravenous drug addictions. The positive rate has positive relation with HCV RNA virus logarithm quantity. Coinfection with HBV are the interfere factor of HCV core antigen detection. In screening experimentations, the detection of HCV core antigen in plasma may be applied as supplement method for anti-HCV-IgG. It can also be used to monitor viremia in HCV infection.