扁平苔藓皮损中肿瘤坏死因子α的表达及其与浸润淋巴细胞间的关系

Expression of tumor necrosis factor alpha and its relationship with infiltrating lymphocytes in lichen planns lesions

目的探讨肿瘤坏死因子-α（TNF—α）在扁平苔藓中的表达及其与淋巴细胞浸润的相关性。方法采用sP免疫组织化学技术对60例扁平苔藓皮损及20例正常皮肤中TNF—α进行检测，并记录每个视野中淋巴细胞浸润数量。结果扁平苔藓皮损中TNF-α阳性表达率为72％，阳性染色主要位于棘细胞膜、真皮浸润的淋巴细胞胞质或胞膜；20例正常人皮肤中均为阴性。扁平苔藓皮损中TNF-α阳性表达率高于正常人皮肤（P〈0．01）。扁平苔藓皮损中TNF—α的表达与患者的年龄、性别及病程无相关性（P值均〉0．05）。经Spearman等级相关分析，扁平苔藓皮损TNF-α仪的表达与淋巴细胞浸润的数量呈正相关（rs=0．47，P〈0．01）。结论TNF-α阳性表达在扁平苔藓的发病中可能起一定作用。

Objective To investigate the expression of tumor necrosis factor alpha （TNF-α） and its relationship with infiltrating lymphocytes in lichen planus （LP）. Methods Tissue specimens were obtained from the lesions of 60 patients with LP and normal skin of 20 human controls. Immunohistoehemical SP method was used to detect the expression of TNF-α, and infiltrating lymphocytes were counted in TNF-α-positire tissue sections. Results TNF-α was expressed in 72% of the LP specimens but in none of the control specimens （P 〈 0.01 ）. Positive staining for TNF-α was mainly located in the membrane of prickle cells, cytoplasm or membrane of dermal infiltrating lymphocytes. The expression of TNF-α in LP was uncorrelated with age, sex or disease course （all P 〉 0.05）, but was positively correlated with infiltrating lymphocyte number （rs = 0.47, P 〈 0.01 ）. Conclusion TNF-α seems to play a certain role in the pathogenesis of LP.