新疆伊犁地区维吾尔、汉族不同人群变应性鼻炎患者IL-4基因甲基化比较研究

Comparative study on IL-4 gene methylation of allergic rhinitis patients from Han and Uighur nationalities in Yili of Xinjiang

目的探讨IL-4基因启动子区甲基化在维吾尔、汉族两个民族变应性鼻炎(AR)患者中的差异。方法采用甲基化特异性聚合酶链反应(MSP),病例组维吾尔族、汉族AR患者各31例,健康对照组维吾尔族、汉族各31例,检测其外周血IL-4基因甲基化状态。结果维吾尔族、汉族AR患者IL-4甲基化率均高于健康对照组,有统计学意义(P<0.05);维吾尔族、汉族AR患者IL-4甲基化率相比较,无统计学差异(P>0.05)。维吾尔族、汉族健康对照组未甲基化率相比较,有统计学差异(P<0.01)。结论 IL-4基因高甲基化是导致AR患病的一个重要原因.汉族人群比维吾尔族易患AR。

To explore the differences of IL-4 gene promoter methylation in allergic rhinitis（AR） patients from Uighur and Han populations,we used methylation specificity polymerase chain reaction（MSP） to exam the methylation degree in 31 cases of Uighur AR patients,31 cases of Han AR patients,31 cases of healthy Uighur people,and 31 cases of healthy Han people.Compared with healthy individuals both from Uighur or Han populations,all AR patients demonstrated higher methylation of IL-4 gene（P 0.05）.But there was no significant deference in IL-4 gene methylation between Han and Uighur AR patients（P 0.05）.Furthermore,health Han and Uighur individuals showed different IL-4 gene methylation（P 0.01）.All the results indicate that high degree methylation of IL-4 gene may is a crucial pathogeny of AR,and Han population is more susceptible to AR than Uighue population.