摘要
小桐子(Jatropha curcas)适应性强,不择土壤,种子油脂性能适宜生物柴油的生产,是重要的生物柴油植物。基于小桐子种子发育过程中的EST序列,采用生物信息学方法,从4640个EST非冗余序列上鉴别了1009个SSR位点并分析其分布特征;开发了11对多态的EST-SSR分子标记,并利用这些分子标记调查了24个不同地理种源的遗传多样性,从每个位点的等位基因数目(2～3,平均为2.45)、期望杂合度(He为0.0887～0.5128,平均是0.2736)、多态信息含量(PIC为0.0847～0.4031,平均是0.2313)等方面反映了小桐子种质的遗传多样性低。进一步分析显示不同地理种源的遗传关系缺乏明显的地理结构。作者开发的EST-SSR分子标记不仅有助于小桐子种质的遗传多样性研究,也有助于小桐子种质间的遗传关系鉴别。
Jatropha curcas （Euphorbiaceae） has created tremendous interest all over the world for the use of its seed oil as a commercial source of biodiesel. Based on 9843 ESTs available from the developing seeds of Jatropha curcas, we identified 1009 SSRs in 4640 unigenes and developed 11 polymorphic EST-SSR markers which exhibited a low level of genetic diversity among germplasms, i.e. allele number varied from 2 to 3, with a average of 2.45 ; Heterozygosity （He） ranged from 0. 0887-0. 5128, with a average of 0. 2736; Polymorphic Information Content （PIC） ranged from 0. 0847-0. 4031, with a average of 0. 2313. Further, we analyzed the genetic relationships among 24 germplasms collected from different areas in southern China, northern Vietnam, and India using the 11 EST-SSR markers. The results showed that there was no a geographic pattern of genotypes across the collection areas of Jatropha curcas. The EST-SSR markers developed in current study is useful for both genetic diversity analysis and identification of genetic relationships among germplasms in Jatropha curcas.
出处
《植物分类与资源学报》
CAS
CSCD
北大核心
2011年第5期529-534,共6页
Plant Diversity
基金
国家自然科学基金项目(30871548)
