摘要
该文以抗除草剂(CP4-EPSPS)转基因大豆GTS-40-3-2为材料,在Lectin基因的扩增体系中设置不同浓度梯度的三氯甲烷、乙醇,模拟这2种有机试剂残留,探讨三氯甲烷、乙醇在转基因成分检测中的抑制作用。结果表明:在PCR反应体系中三氯甲烷的摩尔浓度≥3.77mol/L时,能完全抑制PCR反应;乙醇的摩尔浓度>1.39 mol/L时,PCR反应彻底被抑制。
The different concentration of chloroform and ethanol were designed in the PCR of lectin gene of genetically modified soybean（GTS-40-3-2） in order to investigate the inhibitory effects of chloroform and ethanol on the quality PCR of genetically modified organism detection.The results showed PCR was inhibited when the concentration of chloroform was greater than or equal to 3.77 mol/L and when that of ethanol was greater than 1.39 mol/L in the reaction system.
出处
《中国测试》
CAS
2011年第5期56-58,共3页
China Measurement & Test
基金
国家转基因重大课题(2008ZX08012-001)
四川省农业科学院青年基金(2009QNJJ-037)
