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酿酒酵母ADH2基因沉默表达载体的构建 被引量：2

Constructing the alcohol dehydrogenase ADH2 gene silencing expression vector for Saccharomyces cerevisiae
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摘要 利用重叠延伸PCR获得酿酒酵母基因沉默组件SADH2,经酶切与酿酒酵母穿梭质粒pYES2.0连接,构建酿酒酵母ADH2基因沉默表达载体。经酶切及测序鉴定成功构建了ADH2基因沉默表达载体pSADH2。 It was to synthesize the silencing gene SADH2 of Saccharomyces cerevisiae with overlapping PCR.The silencing gene SADH2 was inserted in pYES2.0 to construct S.cerevisiae ADH2 gene silencing expression vector.Sequencing results showed that the expression vector pSADH2 was correct.
作者 林燕环 魏芳 叶冰莹 张积森 陈由强
机构地区 福建师范大学生命科学学院/农业部甘蔗遗传改良重点开放实验室
出处 《亚热带农业研究》 2011年第3期197-200,共4页 Subtropical Agriculture Research
基金 现代农业产业技术体系建设专项资金资助(CARS-20)
关键词 酿酒酵母 乙醇脱氢酶 pYES2.0 重叠延伸PCR Saccharomyces cerevisiae alcohol dehydrogenase pYES2.0 overlap PCR
分类号 Q939.5 [生物学—微生物学]
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参考文献6

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同被引文献18

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亚热带农业研究
2011年 第3期

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