谷氨酸锌螯合物的制备及测定新方法

Preparation of zinc-glutamate chelate and the new method for mensuration

以谷氨酸和硫酸锌为原料,制备谷氨酸锌螯合物。用有机溶剂沉淀法,以螯合率为指标,筛选最佳螯合及分离提纯条件。结果表明,最佳螯合条件为:pH 6.5,螯合温度90℃,反应时间30 min;分离最佳条件为:将甲醇与螯合浓缩液按10∶1的比例混合提取谷氨酸锌螯合物,用20%的硫酸溶解该螯合物。试验证明,该条件下谷氨酸螯合锌螯合率可达80%以上。此外,定性试验结果均表明谷氨酸与硫酸锌发生了螯合反应。说明该试验条件下可制得稳定且具较高螯合率的谷氨酸锌产品。

Zinc-glutamate chelate was synthesized from glutamic acid and zinc sulfate.Highly pure solid chelates of amino acids with zinc were separated using precipitation fractionation by adding organic solvent.The optimum manufacture conditions for chelates synthesis were evaluated based on the chelation rate.The result showed that the best conditions of chelate were pH 6.5,chelation could take place at 90℃ for 30 min.The optimum conditions of determine were as follows： carbinol and concentrated chelate solution with 10∶1 apart the highly pure chelates of zinc-glutamate chelate,dissolved by 20% sulfurcacid.The chelation rate was up to 80%.And the qualitative test all showed the reaction of chelation take place.This test showed that synthesized conditions could preparation zinc-glutamate chelate with high chelation rate and stability.