摘要
目的测定雷公藤内酯醇和内酯酮的含量。方法采用高效液相色谱法(HPLC法),色谱柱:Diamonsil C18(2)柱,流动相:乙腈-水[25∶75(10 min)→45∶55(15 min)→45∶55(20 min)],流速:1mL/min,检测波长:218 nm。结果在此色谱条件下,2种成分可以完全分离;雷公藤内酯醇、雷公藤内酯酮的线性范围为:0.5625~11.25μg/mL(r=0.9998),0.65~13.0μg/mL(r=0.9999);雷公藤内酯醇、内酯酮平均回收率为98.13%、98.58%;RSD为1.87%、1.92%。结论 HPLC法可简便、准确、快速地测定雷公藤内酯醇和内酯酮的含量。
Objective To establish a HPLC method for determination of triptolide and triptonide in root extracts of Tripterygium wilfordii.Methods The separation was performed on a column of Diamonsil C18(2) with gradient elution of acetonitrile-water [25:75(10 min)→45:55(15 min)→45:55(20 min)] as mobile phase,flow rate was 1 mL·min-1,and the detective wavelength was 218 nm.Results The two components were completely separated under the chromatographic condition,the linear range of triptolide was 0.5625-11.25 μg·mL-1(r=0.9998),and the average recovery was 98.13%(RSD=1.87%).The linear range of triptonide was 0.65-13.0 μg·mL-1(r=0.9999),and the average recovery was 98.58%(RSD=1.92%).Conclusion The method is simple,accurate,rapid and suitable for determination of triptolide and triptonide in root extracts of Tripterygium wilfordii.
出处
《福建中医药大学学报》
2011年第5期27-29,共3页
Journal of Fujian College of Traditional Chinese Medicine
基金
福建省科技厅重大专项(2009YZ0001-1-1)
福建省科技计划项目(2010Y2004)
福建省卫生厅资助课题(WZZY0902)