中国荷斯坦奶牛STAT1全长基因的克隆与序列分析

Cloning and analysis of full length sequence of STAT1 gene in Chinese Holstein dairy cow

根据GenBank中收录哺乳动物STAT1(signal transducers and activators of transcription 1)的直系同源基因序列设计了用于中国荷斯坦奶牛STAT1 cDNA末端快速扩增的引物,并通过SMART法克隆了STAT1全长基因。序列分析发现,STAT1基因在5′和3′非翻译区均存在mRNA的选择性剪接。获得的STAT1基因编码序列长为2 250 bp,编码749个氨基酸。蛋白氨基酸同源性分析表明,STAT1在进化上相对保守,牛STAT1基因与羊、恒河猴、人、猩猩、猪等基因相应序列的同源性分别为99.6%,96.4%,95.87%,95.73%和95.64%。中国荷斯坦奶牛STAT1全长基因的成功克隆,为进一步研究牛STAT1的基因结构、基因表达与调控奠定了基础。

The full length sequence of STAll （signal transducers and activators of transcription 1 ） gene in Chinese Holstein dairy cow was cloned by SMART technology. The primers used for RACE were designed from the sequence of mammalian orthologs of STAT1 genes deposited in GenBank. Sequence analysis revealed that STAT1 gene was selectively spliced in the 5＇ and 3＇ untranslated regions. The Chinese Holstein dairy cow STAT1 gene contains the 2250 bp open reading frame, encoding a putative protein with 749 amino acids. Phylogenetie tree analysis showed that the protein exhibits a high homology with that from sheep （99.6%） , rhesus monkey （96.4%）, human （95.87%）, pongo （95.73%） and pig （95.64%）. The results have laid a foundation for further analysis of structure, expression and regulation of STAT1 gene in cow.