摘要
采用新型直链淀粉-三(5-氯-2-甲基苯基氨基甲酸酯)涂敷型手性固定相在反相色谱条件下拆分戊唑醇对映体,评价了流动相组成(甲醇/水,乙N/水)及柱温(5~45℃)对对映体分离的影响。结果表明:以甲醇/水为流动相时,在甲醇含量100%至40%范围内,戊唑醇对映体均无分离趋势;而用乙腈/水为流动相时,在乙腈含量100%至40%范围内,戊唑醇对映体均可实现基线分离;在5~45℃,V(乙腈):V(水)=1:1,对映体的保留因子(☆)与分离因子(OL)随温度升高而降低,分离度(R。)却先升后降,基于线性Van’tHoff曲线的热力学参数证明戊唑醇对映体的分离受焓驱动。
The separation of tebuconazole enantiomers was performed by high-performance liquid chromatography (HPLC) on the amylose-tris (5-chlorine-dimethy-phenylcarbamate) chiral stationary phase using reverse phase mode. The effects of different mobile phase compositions (methanol/water, acetonitrile/water) and column temperature (5 -45 ℃ ) on the chiral separation were investigated. The results showed that tebuconazole enantiomers did not separate using methanol/water as mobile phase with the content of methanol ranging from 100% to 40%. In contrast, baseline separation of enantiomers was achieved using acetonitrile/water as mobile phase with the content of acetoni- trile ranging from 100% to 40%. The retention factors (k) and selectivity factor (a) decreased when the tempera- ture increased from 5℃ to 45℃, while the resolution (Rs) of enantiomers first increased and then decreased within the same range of temperature (acetonitrile/water,50/50, V/V). Thermodynamical parameters illustrated that separation of tebuconazole enantiomers was driven by enthalpy.
出处
《浙江农业学报》
CSCD
北大核心
2011年第5期932-936,共5页
Acta Agriculturae Zhejiangensis
基金
国家自然科学基金(20807038)
浙江省农业科学院科技创新提升工程资助项目(2007R19Y01E02)
浙江省重大项目(2007C02002)
关键词
戊唑醇对映体
反相色谱
手性拆分
tebuconazole enantiomers
reverse phase chromatography
chiral separation