期刊文献+

新型直链淀粉手性固定相拆分戊唑醇对映体 被引量:1

Enantiomeric separation of tebuconazole by new type of amylose chiral stationary phase
下载PDF
导出
摘要 采用新型直链淀粉-三(5-氯-2-甲基苯基氨基甲酸酯)涂敷型手性固定相在反相色谱条件下拆分戊唑醇对映体,评价了流动相组成(甲醇/水,乙N/水)及柱温(5~45℃)对对映体分离的影响。结果表明:以甲醇/水为流动相时,在甲醇含量100%至40%范围内,戊唑醇对映体均无分离趋势;而用乙腈/水为流动相时,在乙腈含量100%至40%范围内,戊唑醇对映体均可实现基线分离;在5~45℃,V(乙腈):V(水)=1:1,对映体的保留因子(☆)与分离因子(OL)随温度升高而降低,分离度(R。)却先升后降,基于线性Van’tHoff曲线的热力学参数证明戊唑醇对映体的分离受焓驱动。 The separation of tebuconazole enantiomers was performed by high-performance liquid chromatography (HPLC) on the amylose-tris (5-chlorine-dimethy-phenylcarbamate) chiral stationary phase using reverse phase mode. The effects of different mobile phase compositions (methanol/water, acetonitrile/water) and column temperature (5 -45 ℃ ) on the chiral separation were investigated. The results showed that tebuconazole enantiomers did not separate using methanol/water as mobile phase with the content of methanol ranging from 100% to 40%. In contrast, baseline separation of enantiomers was achieved using acetonitrile/water as mobile phase with the content of acetoni- trile ranging from 100% to 40%. The retention factors (k) and selectivity factor (a) decreased when the tempera- ture increased from 5℃ to 45℃, while the resolution (Rs) of enantiomers first increased and then decreased within the same range of temperature (acetonitrile/water,50/50, V/V). Thermodynamical parameters illustrated that separation of tebuconazole enantiomers was driven by enthalpy.
出处 《浙江农业学报》 CSCD 北大核心 2011年第5期932-936,共5页 Acta Agriculturae Zhejiangensis
基金 国家自然科学基金(20807038) 浙江省农业科学院科技创新提升工程资助项目(2007R19Y01E02) 浙江省重大项目(2007C02002)
关键词 戊唑醇对映体 反相色谱 手性拆分 tebuconazole enantiomers reverse phase chromatography chiral separation
  • 相关文献

参考文献14

二级参考文献39

共引文献46

同被引文献5

  • 1成芳,邱进坤,阮源萍.Chiralcel OD-RH手性柱拆分安息香对映体的热力学研究[J].分析科学学报,2007,23(3):253-256. 被引量:3
  • 2Ikai T, Okamoto Y. Structure control of polysaccharide deriv- atives for efficient separation of enantiomers by chromatogra- phy [J]. Chem. Rev. 2009, 109 (11 ): 6077-6101.
  • 3Liu Y, Zou H. High-performance liquid chromatographic evaluation of a coated cellulose tris (3,5-dimethylphenylcar- bamate) chiral stationary phase having a small-pore silica support [J]. J. Chromatogr. A, 2008, 1178( 1-2): 118-125.
  • 4Lammerhofer M. Chiral recognition by enantioselective liq- uid chromatography: Mechanisms and modem chiral station-ary phases [J]. J. Chromatogr. A, 2010, 1217(6): 814-856.
  • 5Cirilli R, Ferretti R, Gallinella B, De Santis E, Zanitti L, La Torre F. High-performance liquid chromatography enan- tioseparation of proton pump inhibitors using the immobi- lized amylose-based Chiralpak IA chiral stationary phase in normal-phase, polar organic and reversed-phase conditions [J]. J. Chromatogr. A, 2008, 1177(1 ): 105-113.

引证文献1

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部