豌豆EST-SSR标记在蚕豆中的通用性与应用

Transferable analysis of pea EST-SSRs on faba bean and its application

对豌豆表达序列标签-微卫星(EST-SSR)分子标记在蚕豆中的通用性与应用进行研究,以期为蚕豆开发出新的分子标记.结果表明:163对豌豆EST-SSR引物中有99对可在蚕豆中获得有效扩增,其中36对引物呈现明显的多态性,共检测到等位位点148个,平均每个位点的等位基因变异数为4.1个;各多态性引物的多态信息量(PIC)值变化范围为0.035到0.810,平均PIC值为0.483 4;实际观察杂合度(HO)变化范围为0.000 1到0.700 0,平均值为0.210 3;期望杂合度(HE)变化范围为0.035 7到0.845 2,平均值为0.538 8;主成分分析与非加权配对算术平均法(UPGMA)聚类分析表明,30份蚕豆种质资源可明显分成2大类群.以上结果表明,本研究所开发的豌豆EST-SSR标记在蚕豆上具有通用性和多态性,可为今后蚕豆遗传多样性、种质资源保存、比较作图和分子标记辅助育种提供新的研究依据.

In order to develop novel molecular markers for faba bean（Vicia faba L.）,the transferability of pea（Pisum sativum L.） EST-SSR（expressed sequence tag-simple sequence repeats） markers and their application were studied in faba bean germplasms.The results revealed that 99 of 163-pair primers for pea EST-SSRs achieved the effective amplification in faba bean,indicating a significant transferability（60.74%） of pea EST-SSR primers to faba bean.Thirty-six of these transferable markers exhibited polymorphism and totally 148 alleles were detected with an average variation of 4.1.The polymorphism information contents of polymorphic markers varied from 0.035 to 0.810 with an average of 0.483 4,while observed heterozygosity（HO） ranged from 0.000 1 to 0.700 0 and expected heterozygosity（HE） ranged from 0.035 7 to 0.845 2,with an average of 0.210 3 and 0.538 8 respectively.Furthermore,UPGMA（unweight pair group method with arithemetic averages） analysis and principal coordinate analysis（PCA） revealed that the 30 germplasms were classified into two major clusters.Because of their polymorphism and transferability,the developed markers in this study will provide the valuable tool for genetic diversity,resource conservation,comparative mapping and marker-assisted breeding of faba bean in the future.