连翘不同提取液对大肠杆菌体外抑菌作用比较

Comparison of antibacterial effects of ethanol extracts from forsythia suspensa on Escherichia coli in vitro

目的了解连翘不同提取液在体外对大肠杆菌的抑菌效果。方法将连翘洗净、烘干、粉碎成粗粉,分别用水、30%乙醇、50%乙醇、70%乙醇9、5%乙醇连续回流提取,提取液浓缩成相当于原药材1 mg/ml的药液。将药液处理后,制成含药滤纸片,采用纸片法,以抑菌环大小为参考指标,观察抑菌效果。结果各提取液均有不同程度的抑菌效果,但50%乙醇提取物对大肠杆菌体外抑菌效果最明显。结论连翘的50%乙醇提取液对大肠杆菌的体外抑菌效果优于其他提取液。

Objective To investigate the antibacterial effects of ethanol extracts from forsythia suspensa on Escherichia coli in vitro.Methods Forsythia suspensa was washed,dried and crushed into coarse powder.It was extrated by the continuous circumfluence with water,30% ethanol,50% ethanol,70% ethanol and 95% ethanol,respectively,and then the different extracts were concentrated into the solutions equivalent to 1 mg / ml of the raw material.These solutions were filtrated and sterilized,and then dropped on filter paper.According to the size of the antibacterial annulus,the antibacterial effects were determined by using paper disk method.Results Different ethanol extracts from forsythia suspensa all showed antibacterial effects on Escherichia coli,but the best effects were achieved by using 50% ethonal.Conclusion The 50% ethanol extracts from forsythia suspensa has a better antibacterial effects on Escherichia coli than other extracts in vitro.