葡萄糖氧化酶修饰聚二甲基硅氧烷-纳米金电极的制备及其应用

Preparation and Application of PDMS-AuNPs Electrode Modified with Glucose Oxidase

利用聚二甲基硅氧烷-纳米金表面生物亲和性结合葡萄糖氧化酶,制备了葡萄糖氧化酶修饰的聚二甲基硅氧烷-纳米金软电极,并用计时安培法在磷酸盐缓冲体系中研究其对葡萄糖的催化氧化,实验结果表明该酶电极对葡萄糖具有明显的催化能力且在葡萄糖浓度为0.66 mM～100 mM的范围内,响应电流与葡萄糖浓度呈良好的线性关系。

In this research,we prepared a flexible GOD-PDMS-AuNPs electrode based on good biocompatibility of the PDMS-AuNPs surface.The catalytic performance of glucose in PBS buffer solution was studied with chronoamperometry.The results showed this modified electrode with a significant catalytic ability for glucose and the response current was linear with glucose concentrations in the range of 0.66 mM～100 mM.