肉色曲霉产碱性脂肪酶发酵条件的研究

Study on Fermentation Conditions for Alkaline Lipase Production by Aspergillus Carneus

对肉色曲霉产碱性脂肪酶的发酵条件进行优化,摇瓶实验表明,该菌株最适产酶培养基组成为(%):黄豆饼粉2,NH4NO3 0.5,玉米粉1,糊精1,柠檬酸钠0.1,K2HPO4 0.5,FeSO4 0.006,大豆油0.6,吐温-800.5mL,pH 9.0.最适产酶温度为28℃,产酶高峰出现在96小时,最佳装液量为35mL,碱性脂肪酶菌株的酶活可达9.3U/mL.脂肪酶的最适作用pH为9.0,最适作用温度为30℃.

By single factor experiment the conditions for the alkaline lipase produced by Aspergillus carneus were optimized.The results showed that the optimal medium for enzyme production was（%）：bean flour 2,NH4NO3 0.5,corn flour 1,dextrin 1,sodium citrate 0.1,K2HPO4 0.5,FeSO4 0.006,bean oil 0.6,Tween-80 1,pH 9.0.The optimal fermentation conditions are at 28℃ for 96h,and the optimal volume of medium is 35mL in 250mL shake flasks.The enzyme activity was 9.3U/mL.The optimal temperature and pH of the lipase are 30℃,pH 9.0.