HPLC方法研究土霉素在南美白对虾体内药物代谢及残留消除规律

Pharmacoknetics and Residues of Oxytetracycline in Penaeus Vannamei by HPLC

用高效液相色谱法研究南美白对虾口服土霉素药饵给药后,在血液、肌肉和肝脏中的残留及消除规律。研究表明:采用Agilengt 1100型液相色谱仪可测定南美白对虾各组织中药物含量,口服给药后0.5 h肝胰腺中土霉素浓度即达到峰值,2h～12h之间肌肉、血液的浓度含量维持在一个较高的水平;代谢到96h(4天)时肌肉、血液、肝胰腺内土霉素含量已降低到检测限(0.01μg/g)以下。本方法简便快速、检出限低、定量准确、精密度高。

High performance liquid chromatography vannamei after oral administration of oxytetracycline bait,blood,muscle and liver residue and elimination.The results show that： The Agilengt 1100 High performance liquid chromatograph can be determined based Penaeus drug content of the tissue,the liver 0.5 h after oral administration of oxytetracycline concentration in the pancreas that peak,2h ~ 12 h between the muscles,the blood concentration content remained at a high level;To 96h（4 days） when the muscles,blood,liver and pancreas oxytetracycline content has been reduced to the detection limit（0.01μg / g） below.The method is simple,rapid,low detection limit,accurate,high precision.