DNA甲基转移酶 凋亡抑制蛋白在肺癌组织中的表达及意义

Expression of DNMT1,DNMT3β and IAP2 in lung carcinoma

目的观察DNA甲基转移酶(DNMT)1、DNMT3β和凋亡抑制蛋白-2(IAP2)在肺癌组织中的表达及与肺癌的临床病理特征关系。方法采用组织微阵列和免疫组织化学技术检测DNMT1、DNMT3β在60例肺癌组织及30名健康人正常肺组织中的表达。结果肺癌组织中DNMT1、DNMT3β、IAP2表达的阳性率均高于正常对照组(P<0.01);3种蛋白的表达在性别、吸烟史、年龄、淋巴结转移、肿瘤分期之间差异无统计学意义(P>0.05);DNMT1、DNMT3β表达与IAP-2的表达明显相关(P<0.01)。结论 DNMT1、DNMT3β表达升高是肺癌形成、发展中的普遍事件,可作为诊断的有效指标,DNMT1与DNMT3β的表达可能存在共同调节机制;且可能参与了凋亡抑制蛋白IAP2调节作用。

Objective To evaluate the significance of aberrant DNA methyltranaferase（DNMTs） and IAP2 expression in human lung carcinoma.Methods Immunohistochemistry was used to determine protein expression of DNMTs 1,3β and IAP2.Results The positive expression rate of DNMT1,DNMT 3β and IAP2 in lung cancer were all significantly higher than those in normal（P0.05）.There was no significant correlation between the expression of DNMT1,DNMT 3β,IAP2 and the clinicopathological parameters of tumors（sex,age,smoke,lymphatic metastasis,clinical stage,P0.05）.The positive expression rate of DNMT1 and DNMT 3β had positive correlation with the expression of IAP2（P 0.01）.Conclusion Over-expression of DNMT1,DNMT 3β and IAP2 are involved in human lung carcinoma.There could be a certain coregulation mechanism between DNMT1 and DNMT 3β which could regulate cIAP2.