柱前衍生化-HPLC法测定患者体内丙戊酸钠的血药浓度

Determination of the concentration of sodium valproate in patients' serum by precolumn derivatization-HPLC method

目的建立反相高效液相色谱法测定人血清中丙戊酸钠血药浓度。方法血清用环己烷提取,以环已烷羧酸为内标,2-溴苯乙酮为衍生化试剂,用高效液相色谱法测定丙戊酸钠血药浓度。采用Symmetry C18(4.6 mm×250 mm,5μm)色谱柱;流动相为甲醇-水(82∶18);检测波长为248 nm;流速为1.0 ml.min-1;柱温:30℃。结果血清中丙戊酸钠线性范围为12.5～150mg.L-1,平均回收率98.74%,日内RSD<5%。结论该法快速、灵敏、准确,适用于临床常规监测需要。

Aim To determine sodium valproate in patients' serum by high performance liquid chromatography(HPLC).Methods Serum sample was extracted with n-Hexane.Cyclohexanecarboxylic acid and 2-Bromoacetophenone were selected as an internal standard and derivative reagent respectively.The analytical column was Symmetry C18(4.6 mm×250 mm,5 μm) with the temperature at 30℃.The detection wavelength was 248 nm and the flow rate was 1.0 ml·min-1.Column temperature 30℃.The mobile phase consisted of methanol-water(82:18).Results The linear range of sodium valproate was 12.5~150 mg·L-1,The mean recovery was 98.74%.The relative standard deviation(RSD)of inter-day for sodium valproate was less than 5%.Conclusion The method is rapid,accurate,sensitive and suitable for clinical routine monitoring for sodium valproate.