摘要
建立固相萃取-反相高效液相色谱法测定桂圆中残留嘧菌酯的方法.色谱柱Shim-pack VP-ODS 150mm×4.6mm,检测波长为225nm,流动相为V(乙腈)∶V(水)=75∶25,流速为0.6mL/min,进样量为20μL.嘧菌酯在0.03～12.4μg/mL(r=0.999 4)的质量浓度范围内与峰面积成良好线性关系,检测限为0.01μg/mL,嘧菌酯的回收率为98.72%～100.20%,相对标准偏差2.26%～3.88%.该方法操作简便快速,可作为桂圆中嘧菌酯含量监测的控制方法.
A reversed-phase high performance liquid chromatograghic（HPLC） method with solid-phase extraction was developed for the determination of azoxystrobin in longan.The HPLC conditions were as follows： The column was Shim-pack VP-ODS 150 mm×4.6 mm,V（acetonitrile）∶V（H2O）=75∶25 as mobile phase,flow rate 0.6 mL/min,injection volume 20 μL and detection wavelength at 225 nm.Good linearity of azoxystrobin was obtained over the range of 0.03~12.4 μg/mL（r=0.999 4）.The detection limits were 0.01 μg/mL for azoxystrobin.The average recoveries ranged from 98.72% to 100.20% with a relative standard deviation of 2.26%-3.88%.The method is fast and simple and is recommended for residue detection and analysis of azoxystrobin in longan.
出处
《西南大学学报（自然科学版）》
CAS
CSCD
北大核心
2011年第9期77-80,共4页
Journal of Southwest University(Natural Science Edition)
基金
贵州省遵义市科技局项目([2008]26号)
贵州省教育厅自然科学项目黔教科([2010]085号)
关键词
固相萃取
反相高效液相色谱法
嘧菌酯
桂圆
solid-phase extraction
reversed-phase high performance liquid chromatograghic（RP-HPLC）
azoxystrobin
longan
