高效液相色谱法测定抗乳腺小叶增生合剂中丹参酮ⅡA的含量

Determination of tanshinone ⅡA in Kangruxian Xiaoyezengsheng Mixture by HPLC

目的：建立高效液相色谱法（HPLC）测定抗乳腺小叶增生合剂中丹参酮ⅡA的含量。方法：采用Diamond C18柱（250min×4．6mm，5μm），以甲醇-水（85：15）为流动相，波长270nm，柱温40℃。结果：丹参酮ⅡA在10-120μg／mL范围内呈良好线性关系（r=0．9999，n=6），平均加样回收率为99．89％，RSD=1．17％。结论：本法操作简单、准确、重复性好，可用于抗乳腺小叶增生合剂的质量控制。

Objective ：To establish a HPLC method for the determination of tanshinone Ⅱ A in Kangruxian Xiaoyezengsheng Mixture.Methods：The sample was analyzed in the Diamond C18 column （250 mm×4.6 mm,5 μm）,with the mobile phase of methanol-water （85：15）, using ultraviolet detector at 270 nm and the column temperature of 40 ℃. Results：There was good linearity within the concentration range of 10-120 μg/mL for tanshinone Ⅱ A（r=0.999 9,n=6）.The average recovery rate was 99.89%（RSD=1.17%）. Conclusion：The method is simple, accurate and reproducible,which can be used for the quality control of Kangruxian Xiaoyezengsheng Mixture.