HPLC同时测定莪术油中6种倍半萜成分

Simultaneous Determination of Six Sesquiterpenes in Oleum Curcumae by HPLC

目的:建立反相高效液相色谱法同时测定莪术油中6种倍半萜类(莪术二酮、莪术醇、牻牛儿酮、呋喃二烯、莪术烯、β-榄香烯)成分含量的方法。方法:色谱条件Agilent Extend C18色谱柱(4.6 mm×250 mm,5μm),流动相为乙腈(A)-甲醇(B)-水(C),梯度洗脱(0～20 min,30%～65%A,40%～30%B;21～30 min,65%A,30%B),流速1.0 mL.min-1,柱温30℃,检测波长216 nm。结果:6种倍半萜类化合物莪术二酮、莪术醇、牻牛儿酮、莪术烯、呋喃二烯和β-榄香烯分别在为43.72～2 186,44.37～2 219,15.49～774.4,28.30～1 415,40.13～2 007,16.74～837.0 ng呈现良好的线性关系(r>0.999);平均加样回收率在98.46%～103.9%;6种倍半萜类化合物含量重复性试验RSD<1.29%。结论:方法快速准确、具有良好的重复性和稳定性,可用于莪术油的质量控制。

Objective：To develop an HPLC method for simultaneous determination of six sesquiterpenes（curdione,curcumol,germacrone,curzerene,furanodiene,β-elemene） in Oleum Curcumae.Method：HPLC was performed on an Agilent Extend Cl8 analytical column（4.6 mm × 250 mm,5 μm） with gradient elution（0-20 min,30%-65% A,40%-30% B,21-30 min,65% A,30% B）of acetonitrile（A）-methanol（B）-water（C） at the flow rate of 1.0 mL·min-1.The column temperature was maintained at 30 ℃.Detection wavelength was 216 nm.Result：The six sesquiterpenes had good linearity with correlation coefficient 0.999,ranging from 43.72-2 186,44.37-2 219,15.49-774.4,28.30-1 415,40.13-2 007 ng and 16.74-837.0 ng respectively.The average recoveries were between 98.46%-103.9%,repeatability experiments showed that RSD values of their contents were less than 1.29%.Conclusion：The developed method is rapid,accurate with high repeatability and stability,which is helpful to control the quality of Oleum Curcumae.