HPLC测定双黄连注射液中木犀草苷

HPLC Determination of Luteoloside in Shuanghuanglian Injection

目的:建立双黄连注射液中木犀草苷的RP-HPLC含量测定方法。方法:采用聚酰胺柱纯化富集木犀草苷,通过Waters C18(4.6 mm×250 mm,5μm)色谱柱,以乙腈(A)-0.1%磷酸(B)为流动相,梯度洗脱,流速为1.0 mL.min-1,检测波长350 nm,柱温35℃。结果:木犀草苷进样量在34.3～343.0 ng线性关系良好(r=0.999 7);方法回收率为99.2%,RSD 3.3%(n=6)。结论:方法灵敏、简便、准确,可用于双黄连注射液中木犀草苷的含量测定。

Objective： To establish an HPLC method to determine the content of luteoloside in Shuanghuanglian Injection.Method： Purification and enrichment of luteoloside were performed through polyamide column.The Waters C18（4.6 mm×250 mm,5 μm） column was adopted.The mobile phase consisted of acetonitrile（A）-0.1% phosphoric acid（B） with gradient elution at the flow rate of 1.0 mL·min-1.The detection wavelength was at 350 nm,and the column temperature was set at 35 ℃.Result： The linear range of luteoloside was 34.3-343.0 ng（r=0.999 7）,and the average recovery was 99.2% with RSD of 3.3%（n=6）.Conclusion： This method is sensitive,simple,accurate,and suitable for the determination of the content of luteoloside in Shuanghuanglian Injection.