摘要
目的:建立双黄连注射液中木犀草苷的RP-HPLC含量测定方法。方法:采用聚酰胺柱纯化富集木犀草苷,通过Waters C18(4.6 mm×250 mm,5μm)色谱柱,以乙腈(A)-0.1%磷酸(B)为流动相,梯度洗脱,流速为1.0 mL.min-1,检测波长350 nm,柱温35℃。结果:木犀草苷进样量在34.3~343.0 ng线性关系良好(r=0.999 7);方法回收率为99.2%,RSD 3.3%(n=6)。结论:方法灵敏、简便、准确,可用于双黄连注射液中木犀草苷的含量测定。
Objective: To establish an HPLC method to determine the content of luteoloside in Shuanghuanglian Injection.Method: Purification and enrichment of luteoloside were performed through polyamide column.The Waters C18(4.6 mm×250 mm,5 μm) column was adopted.The mobile phase consisted of acetonitrile(A)-0.1% phosphoric acid(B) with gradient elution at the flow rate of 1.0 mL·min-1.The detection wavelength was at 350 nm,and the column temperature was set at 35 ℃.Result: The linear range of luteoloside was 34.3-343.0 ng(r=0.999 7),and the average recovery was 99.2% with RSD of 3.3%(n=6).Conclusion: This method is sensitive,simple,accurate,and suitable for the determination of the content of luteoloside in Shuanghuanglian Injection.
出处
《中国实验方剂学杂志》
CAS
北大核心
2011年第20期82-84,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
河南省科技厅重点科技公关项目(102102310017)