痰瘀同治方对大鼠颈总动脉粥样硬化易损斑块稳定性的影响

Influence of Tanyu Tongzhi Formula on Stability of Atherosclerotic Vulnerable Plaque of Carotid Artery in Rats

目的:探讨痰瘀同治方对大鼠颈总动脉粥样硬化(AS)易损斑块稳定性的影响。方法:采用高脂、高蛋氨酸喂养,4周末行颈总动脉球囊拉伤术造模,术后将大鼠随机分为空白对照组、模型组、辛伐他汀组、复方维生素组、痰瘀同治方(TYTZ)7.8,3.9,1.95 g.kg-1组,连续ig 8周。实验结束后,测定各组血清同型半胱氨酸(Hcy)、血清基质金属蛋白酶-9(MMP-9),基质金属蛋白酶组织抑制因子-1(TIMP-1)和斑块中MMP-9,TIMP-1阳性表达。结果:与模型组比较,辛伐他汀组、复方维生素组和痰瘀同治方7.8,3.9 g.kg-1组血清MMP-9降低并TIMP-1升高,斑块中MMP-9阳性表达降低。结论:痰瘀同治方可通过调节MMP-9/TIMP-1起到稳定颈总动脉粥样硬化易损斑块的作用。

Objective： To observe the effects of Tanyu Tongzhi（TYTZ） formula on stability of vulnerable atherosclerotic plaque of carotid artery in rats.Method： With high cholesterol lipid and 2% L-methionine feeding for four weeks,the vulnerable atherosclerotic plaque model was reproduced by operation on carotid artery endothelium for inducing sacculus pull injury.Rats were randomized into five groups： normol control group,model group,simvastatin group,vitamin complex group,TYTZ group（7.8,3.9,1.95 g·kg-1） and administrated orally for 8 weeks.At the end,the blood sample was collected to measure the concentration of Hcy,MMP-9 and TIMP-1,the protein contents of MMP-2 and TIMP-2 in plaque were detected by immunohistochemistry.Result：In contrast to model group,TYTZ（7.8,3.9 g·kg-1）,simvastatin and vitamin complex decreased the concentration of serum MMP-9 and increased the concentration of TIMP-1,the expression of MMP-9 detected in plaque was also lower.Conclusion： TYTZ formula could stablize the vulnerable atherosclerotic plaque of carotid artery through mediating MMP-9 and TIMP-1.