大豆LEA基因家族全基因组鉴定、分类和表达

Genome-Wide Identification,Classification and Expression Analysis of LEA Gene Family in Soybean

【目的】鉴定大豆全基因组中的LEA家族基因,对其进行定位、分类以及组织表达分析,为植物LEA基因的功能研究与利用提供基础。【方法】利用大豆基因组数据库,通过生物信息学手段,鉴定并获得大豆LEA家族基因的全序列、定位和拷贝数信息;通过序列比对进行进化和分类分析;利用大豆发育表达芯片数据、NCBI中UniGene的EST表达数据进行组织表达谱分析。【结果】系统地分析鉴定了36个大豆的LEA家族基因,根据结构域的差异和系统发育分析将这些LEA基因分成8个亚家族。基因定位分析结果表明,这些基因分布于大豆的16条染色体上,启动子分析表明,几乎全部LEA基因的启动子区含有逆境反应顺式作用元件。各个发育阶段表达谱的分析结果表明,多数成员至少在一个组织中表达,10个差异表达的基因中有5个在种子发育时期优势表达,另外5个在其它部位优势表达。【结论】通过全基因组扫描,获得大豆基因组的36个LEA家族基因,它们分属于8个不同的亚家族,分布于16条大豆染色体上,启动子区含有逆境相关顺式作用元件,基因表达具有一定特异性。

【Objective】 Identification,phylogenetic and tissue specific expression pattern analysis of LEA family genes in Soybean will be helpful to study the functions of plant LEA genes.【Method】 Based on soybean genome database and bioinformatic method,soybean LEA family genes were identified and their gene sequences,chromosome location and duplication information were obtained.LEA proteins were classified according to their structure and phylogeny relationship.The soybean developmental expression database and EST data from NCBI UniGene were used to analyze the expression pattern of these genes at different development stages.【Result】A total of 36 LEA genes were systematically identified from soybean and classified into 8 subfamilies according to motif analysis and phylogeny relationship.They were located on 16 chromosomes and most of them had stress related cis-acting elements in their promoter regions.Ten differentially expressed genes were found,and five of them were highly expressed in seeds by microarray and EST data at each developmental stages.【Conclusion】Thirty six LEA family genes in soybean were identified by genome-wide screening.They were classified into 8 subfamilies and distributed on 16 chromosomes with stress related cis-acting elements in their promoter regions.They were differentially expressed in different tissues and developmental stages.These results are helpful for their functional analysis and utilization in crop genetic improvement.