不同体重环江香猪胎儿的羊水和尿囊液化学成分分析

Analysis of Chemical Composition in Amniotic Fluid and Allantonic Fluid of Fetus with Different Body Weights in Huanjiang Mini-Pigs

【目的】确定环江香猪妊娠早期胎儿体重与胎液生化参数的关系,为揭示宫内发育迟缓的发生机制及探寻其防治方法提供参考。【方法】选取体重35—40 kg的雌性环江香猪,7头,单栏饲养,每栏1头。配种第45天,颈动脉放血处死并解剖,分别称取胎儿、胎盘和子宫的重量;对每头母猪各选取2只体重最大和最小的胎儿,采集与胎儿对应的母猪羊水、尿囊液和胎盘,-20℃保存备用。【结果】与体重最大胎儿相比,体重最小胎儿的体重及母体胎盘重、子宫重和羊水+尿囊液体积分别低23%、62%、41%和39%(P<0.05),羊水总胆固醇浓度高30%(P<0.05),尿囊液丝氨酸、丙氨酸和酪氨酸浓度分别低58%、44%和29%(P<0.05),而赖氨酸、精氨酸和苏氨酸浓度分别高25%、40%和114%(P<0.05);胎盘总蛋白质含量和诱导型一氧化氮合酶的活性分别高39%和70%(P<0.05),结构型一氧化氮合酶的活性低22%(P<0.05)。【结论】妊娠早期胎儿体重大小与部分生化参数密切相关。

【Objective】 The present study was conducted to investigate the relationship between body weight（BW） of fetus and biochemical parameters of fetal liquid in Huanjiang mini-pigs in order to illuminate the genesis mechanism,and approach prevention and cure methods of intrauterine growth retardation（IUGR）.【Method】 Seven female mini-pigs with a live BW of 35-40 kg were chosen in this study and raised in seven pens of one pig.At the end of a 45-day feeding period after serviced,the sows were killed by bloodletting of arteria carotis,and the BW of fetus and the weight of placenta and womb were got.The amniotic fluid,allantonic fluid and placenta were collected from two fetus with minimal BW or maximal BW per sow,respectively,for analysis.【Result】 Results showed that the BW of fetus,placenta weight,womb weight,volume of amniotic fluid plus allantonic fluid in fetus with minimal BW were lower by 23%,62%,41% and 39%（P0.05）,respectively.The concentration of total cholesterol in amniotic fluid was higher by 30%（P0.05）.The concentrations of serine,alanine and tyrosine in allantonic fluid decreased by 58%,44% and 29%（P0.05）,while of lysine,arginine and threonine increased by 25%,40% and 114%（P0.05）,respectively.The content of total protein and activity of inducible nitric oxide synthetase（NOS） in placenta were higher by 39% and 70%（P0.05）,respectively,while of constitutive NOS decreased by 22%（P0.05）,when compared with the fetus with maximal BW.【Conclusion】 These findings suggested that there is a close relationship between the BW and biochemical parameters of fetus during early pregnancy in Huanjiang mini-pigs.