多柔比星稀土配合物与人血清白蛋白相互作用的研究

Study on the Interaction of Adriamycin Rare Earth Complexes with Human Serum Albumin

运用荧光光谱法研究了多柔比星稀土配合物(ADM-M)与人血清白蛋白(HSA)的相互作用。结果表明:多柔比星稀土配合物对HSA的荧光有较强的猝灭作用,其荧光猝灭为混合猝灭过程。求得多柔比星稀土配合物与HSA的结合常数、结合位点数。由热力学参数确定了多柔比星稀土配合物与HSA的作用力类型。同时用同步荧光光谱法考察了多柔比星稀土配合物对HSA构象的影响。

The interaction of adriamycin rare earth complexes（ADM-M） with human serum albumin（HSA） have been studied by fluorescence spectrum.The results showed that ADM-M strongly quenched the fluorescence produced by HSA and this quenching could be interpreted in terms of mixture quenching.The binding constants and the number of binding sites between them have been measured.Based on the thermodynamic parameter analysis,the main binding force between them was confirmed.Meanwhile,the effect of ADM-M on the conformation of HSA was investigated using synchronous fluorescence spectrum.