羟丙基-β-CD包结荧光法测定大黄素的研究

Determination of Emodin with Hydroxypropyl-β-CD Inclusion Spectrofluorimetry

用荧光光谱研究了羟丙基-β-环糊精(HP-β-CD)与大黄素间的相互作用,发现两者可形成1∶1的超分子包合物,大黄素包合物在λex/λem=467/547nm处发射荧光,包合常数为85.18L/mol。据此建立了羟丙基-β-环糊精增敏测定大黄素的荧光分析新方法。在0.32～3.24μg/mL浓度范围内,大黄素的荧光强度与浓度呈良好的线性关系,相关系数r为0.9977,检出限为0.032μg/mL,相对标准偏差为0.45%。该体系的抗干扰能力及稳定性好,对实际样品进行了测定,回收率为98.46%。

The supramolecular interaction between emodin and hydroxypropyl-β-CD was studied by spectrofluorimetry.The results show that hydroxypropyl-β-CD reacts with emodin to form an inclusion complex with an association constant of 85.18 L/mol and the composition of the complex is 1∶1（HP-β-CD∶Emodin）.Based on the significant enhancement of fluorescence intensity of emodin in the inclusion complex,a spectrofluorimetric method with high sensitivity and selectivity was developed for the determination of emodin in aqueous solution.Under the optimum conditions,the complex exhibited maximum excitation and emission wavelengths at 467 nm and 547 nm,respectively.The linear range of the method was 0.32~3.24 μg/mL with a detection limit of 0.032 μg/mL,and the relative standard deviation was 0.45%.Emodin in real samples was successfully measured with a recovery of 98.46%.