Hedgehog通路对乳腺癌细胞MDA-MB-231c-jun、uPA表达的影响和机制

The effect of hedgehog signaling pathway on c-jun,uPA expression of breast cancer cells MDA-MB-231

目的:研究Hedgehog信号转导通路对人乳腺癌细胞MDA-MB-231c-jun、uPA表达的影响和作用机制,认识其在侵袭转移中的意义。方法:应用Hedgehog信号通路抑制剂Cyclopamine作用人乳腺癌细胞MDA-MB-231,Transwell小室进行侵袭迁移实验;Western blotting检测P-c-jun、uPA蛋白的水平:RT-PCR检测Gli1、c-jun和uPA的mRNA水平。结果:应用Cyclopamine后,细胞的侵袭迁移能力明显下降;P-c-Jun、uPA蛋白表达减少;Gli1、c-jun和uPA mRNA水平降低。结论:Hedgehog信号通路可通过活化c-jun上调uPA的表达,促进乳腺癌细胞MDA-MB-231细胞的侵袭转移。

Objective： To explore the effect and the possible mechanisms of Hedgehog singaling pathway on expression of c - jun, uPA in MDA - MB - 231 cells. Methods： Invasion and metastasis of MDA- MB- 231 cells were evaluated with transwell chamber.The expression of P- c- jun and uPA were detected by Western blot. The mRNA levels of Gli 1, c - jun and uPA were measured by reverse transcriplion - poly - merage chain reaction （ RT - PCR ）. Results ： When MDA - MB - 231 cells were treated with Cyclopamine, invasion and metastasis were depressed. The expression level of P- c- Jun and uPA were decreased. The mRNA levels of Ghl, c-jun and uPA were also reduced. Conclusion： invasion, metastasis and uPA level can be induced by activeled Hedgehog singnaling nathwav with activation of c - iun.