表阿霉素脂质体纳米粒治疗大鼠移植性肝癌的实验研究

Efficacy of Epirubicin-nanoliposome for transplanted liver cancer in rat

目的通过对移植肝癌模型大鼠肝动脉注射表阿霉素脂质体纳米粒,观察表阿霉素脂质体纳米粒对移植性肝癌的治疗效果。方法建立大鼠移植性肝癌动物模型,并将肿瘤模型大鼠随机分为3组,每组20只。大鼠正中开腹,暴露肝脏,游标卡尺测定肿瘤的长短径;肝动脉插管固定;依次分组给药。各组动物肝动脉注药后生存超过一周者,记录生存天数。一周后再次开腹,测定并记录肿瘤的生长率、肿瘤的坏死范围及生命延长率。结果治疗前各组间肿瘤体积无显著性差异(P>0.05),治疗后肝动脉注射N S组,平均生存期为12.7 d;肝动脉注射游离表阿霉素(EPI)组,平均生存期18.7 d;肝动脉注射EPI-PBC A-N P治疗组,平均生存期为32.5d。治疗后各组间肿瘤体积存在差异性(P<0.05),EPI组、EPI-PBC A-N P治疗组动物平均生存天数较N S组均显著延长(均P<0.01)。结论肝动脉注射表阿霉素脂质体纳米粒组肿瘤生长率明显降低、生命延长率明显提高(P<0.01)。

【Objective】 To observe the therapeutic effects against transplanted liver cancer with Epirubicin Nanoliposome via hepatic artery.In presence of magnetic field,the animal bearing transplanted liver tumor was injected Epirubicin Nanoliposome containing Epirubicin in order to observe efficacy of the nanopartlicles for transplanted liver cancer.【Methods】 The rat model of transplanted liver cancer were established.After one week,they were divided into three groups at random.Each group is 20.Abdominal exposure was carried out through a midline abdominal incision.The diameter of tumor was detected and a cannula was inserted into the hepatic artery and fixed up it.The survival days of the animals which survive time was over 7 days post EPI administration was recorded.After one week,abdominal exposure was carried out through a midline abdominal incision again.Volume of tumor,tumor growth rate,degree of tumor necrosis and increasing longer survival（ILS） were detected and investigated.【Results】 Before therapy,no statistic difference was found in each group volume of tumor（P 0.05）.After therapy,statistic difference was found in each group volume of tumor（P 0.05）.Normal Saline treated group,survived time was 12.7 days;free Epirubicin treated group,survived time was 18.7 days.Epirubicin Nanoliposome treated group Survived time was 32.5 days.The survival rate of animals of every group was analyzed with increasing longer survival.It demonstrated statistical difference existed each group.【Conclusions】 Epirubicin Nanoliposome can remarkable increase survival rate of animals,compared to NS treated animals or animals treated with free Epirubicin.