山东省玉米骨干自交系和杂交种的SSR指纹图谱构建

Fingerprinting Establishment of Maize Elite Inbred Lines and Hybrids by SSR Markers in Shandong Province

构建骨干品种(系)指纹图谱,可为新品种的审定和品种保护建立准确可靠的依据,也是适应玉米育种快速发展的需要。分别利用74对SSR引物对35份骨干自交系和19份主栽杂交种进行了SSR分析。筛选出10对和20对适于玉米自交系和杂交种分析的核心引物,分别检测出115和124个等位变异,将扩增条带数字化,构建了自交系和杂交种的指纹图谱,出现相同指纹图谱的概率分别为3.13×10-11和5.59×10-25。构建的指纹图谱用于玉米品种鉴定是可行的,对玉米新品种审定和品种权保护具有重要意义。

Establishment of fingerprinting is accurate and reliable basis for cultivar assessment and new plant variety protection.Thirty-five elite maize inbred lines and 19 hybrids were analyzed with 74 pairs of primers respectively by SSR markers to establish the standard DNA fingerprinting for seed test.Ten and 20 pairs of primers distributed on ten chromosomes in maize genome were finally screened to develop numbered fingerprinting of inbred lines and hybrids.There were 115 and 124 alleles in inbred lines and hybrids fingerprinting respectively.The probability for the fingerprinting of each line and hybrid was 3.13×10-11 and 5.59 × 10-25,which was feasible to be used in variety identification.The established fingerprinting had a tremendous potential in new variety assessment and intellectual property rights protection.