不同培养基和培养条件对脱毒马铃薯快繁生长的影响

Effect of Different Medium and Culture Condition on the Rapid Propagation of Virus-free Potato

为建立一种高质高效和低成本的马铃薯脱毒试管苗快繁技术,试验主要以‘中薯1号’脱毒试管苗为材料,对其快繁技术中培养基和光温条件进行研究。结果表明:在5种培养基中,MS和1/2MS是较优的快繁培养基,试管苗生长快,生长势好。在4种不同光温条件中,高温强光(T3)是最好的快繁条件,而自然光温下阳面房间(T2)可充分利用自然光温,且试管苗生长正常,具有节约能源和低耗的特点。MS、1/2MS与T2、T3的组合中,MS/T3植株生长最旺盛,但所有4个组合间各项生长指标差异均不显著,而1/2MS/T2可充分利用自然光温条件,节省药品,极大节约生产成本,是一种较好的选择。

The objective of this paper is to establish a set of high quality,high effective and lost-cost rapid propagation production technology about the virus-free potato plantlet.This experiment investigated that the effects of different mediums and light-temperature condition on virus-free potato rapid propagation used the ＇ Zhongshu1 ＇.The results showed that the MS and 1/2MS was the most suitable for rapid propagation because of their well and vigorous growth among 5 mediums.The high temperature and intense light（T 3） was the best condition for rapid propagation among 4 different light-temperature conditions,and the sunny side lab room（T 2） was preferable for rapid propagation because of adequate utilization of natural light-temperature.The MS/T 3 appeared the best performance while there were no significant differences in the growth index among the 4 combinations.However,the 1/2MS/T 2 was the preferable substitute because it could adequately utilize the natural light-temperature and reduce the cost.