多孔硅支撑磷脂双层膜的制备与表征

Preparation and Characterization of Porous Silicon-Supported Bilayers Lipid Membranes

制备了一种以多孔硅为基底的新型支撑磷脂双层膜，多孔硅具有良好的光反射干涉性能，在多孔硅表面构筑磷脂双分子层膜有望形成一类新型的生物传感器。通过电化学刻蚀法制各了孔径为35～45nm，孔道深度约为15μm的多孔硅，并用电子显微镜观测了其孔道结构，经原子力显微镜检测，其表面均方根粗糙度（RMSR）仅为0．64nm。用挤出法制备了粒径约为1541nm的DOPC脂质体，并通过融合法使其在高亲水性的多孔硅基底表面沉积形成支撑磷脂双层膜。采用原子力显微镜（AFM）观察了膜表面，测得磷脂膜的厚度为3．87nm。同时利用多孔硅的光反射干涉特性，检测到多孔硅支撑磷脂双层膜对磷酸缓冲液中离子具有排阻作用，进一步验证了膜的形成。

A new kind of supported lipid bilayer membrane based on porous silicon was prepared. Porous silicon has an optical characterization of reflective interference, which can make it as a new kind ofbiosensor if coated with bilayers lipid membrane on the surface. The porous silicon with typical pore size of35-45 nm in diameter and 15 /am in depth was prepared by electrochemical etching. Atomic force microscopy （AFM） measurements were carried out to observe the morphology of the sample surface and the results showed that the Root Mean Squared Roughness（RMS） was only 0.64nm.The DOPC liposomes with a mean diameter of 154.1 nm were prepared by extrusion, and the supported bilayers lipid membranes were formed on highly hydrophilic porous silicon with the liposome solution by fusion method. The AFM measurements showed that the lipid bilayer membrane formed on the porous silicon＇s surface and the thickness of the membrane was about 3.87nm. Furthermore, the formation of the membrane was confirmed by testing the ability of the porous silicon supported membrane to exclude the ions based on the reflective interference of porous silicon.