喷雾干燥法制备植物乳杆菌MA2发酵剂

Starter Lactobacillus plantarum MA2 Preparation Technology by Using Spray Dying Methord

对一株Kefir源植物乳杆菌MA2喷雾干燥法制备发酵剂的工艺进行初步研究,通过正交实验对保护剂的配方进行优化,确定保护剂的配方为:谷氨酸钠20,g/L,海藻糖50,g/L,乳糖50,g/L,脱脂乳100,g/L.通过响应面分析方法对喷雾干燥的条件进行优化,确定喷干最适条件为:进口温度151.63,℃,进料流量62.07,mL/h,保护剂与菌泥比例(g∶L)3.38∶1.按上述条件得到干菌粉的活菌数为2.11×109 g–1.4,℃冷藏保存半年后活菌数仍能达到1.02×109 g–1,凝乳时间14,h,胆固醇移除率32.0%,为实现乳酸菌发酵剂的高效生产提供实验基础.

The Lactobacillus plantarum starter preparation technology by using spray dying methord was studied. The starter was Lactobacillus plantarum MA2 isolated from Kefir grains. The protectants used in spray dying were optimized by orthogonal experiment and the optimum properties of protectants were as follows： sodium glutamate 20 g/L, trehalose 50 g/L, lactose 50g/L and skim milk 100 g/L. The conditions of spray dying were optimized by response surface analysis, and the best conditions were as follows： inlet temperature 151.63℃, injective flow of mixture 62.07 mL/h, proportion of protec- tant and cell 3.38 ： 1. The Lactobacillus plantarum powder has prepared by spray dying. The number of live bacteria is 2.11 - 109 g 1. After cold storage at 4 ℃ for half year, the living bacteria amount of bacterium powder is 1.02 ×10^9g-1, the time of milk-solidfying was 14 h, and the percentage of cholesterol removed was 32.0%. This result achieved the intended purpose of efficient preparation the fermentation starter.