不同预处理方法对棉秆酶水解的影响

Effects of Different Pretreatment Methods on Enzymatic Hydrolysis of Cotton Stalk

研究稀硫酸法、亚硫酸法、亚硫酸盐法预处理的化学药品添加量对棉秆酶水解的影响,对预处理前后的棉秆进行扫描电镜观察,并对3种方法进行了比较.在固液比1∶4、温度180,℃、保温20,min的预处理条件下,纤维素酶用量(相对于绝干底物)10 U/g、纤维二糖酶用量(相对于绝干底物)3.6 U/g的酶水解条件下,稀硫酸法预处理在98%浓硫酸添加量为5.52%时,棉秆的酶水解转化率为42.63%;亚硫酸法预处理在亚硫酸添加量7%时,棉秆的酶水解转化率为81.25%;亚硫酸盐法预处理在98%浓硫酸添加量0.92%、亚硫酸氢钠添加量为8%时,棉秆的酶水解转化率为70.06%.

Effect of the chemicals dosage of dilute sulfuric acid, sulfurous acid and sulfite pretreatment and enzymatic hydrolysis of cotton stalk was investigated. The scanning electron microscopic （SEM） analysis of untreated and treated cotton stalk was performed for comparing these methods. Under conditions of liquid ratio 1 ： 4, temperature 180 ~C for 20 min, cellulase 10 U/g,cellobiase 3.6 U/g compared to substrate, enzymatic hydrolysis conversion rate was 42.63% when 5.52% sulfuric acid with mass fraction of 98% for pretreating cotton stalk, enzymatic hydrolysis conversion rate was 81.25% when 7% sulfurous acid for pretreating cotton stalk, and enzymatic hydrolysis conversion rate was 70.06% when 0.92% sulfuric acid with mass fraction of 98% and 8% sodium bisulfite for pretreating cotton stalk.