乙肝表面抗体定性检测S/Co值的临床意义

目的:寻找乙肝表面抗体定性检测S/CO值与定量值之间的关系。方法:不同浓度的临床标本分别由时间分辨荧光免疫分析法(TRF)及酶联免疫吸附试验(ELISA)检测两种方法来检验,根据检测结果绘制散点图,使用SPSS软件进行数据分析。结果:ELISA检测出的S/CO值可以通过转换方程式Y=26.02×e0.128x转换成TRF定量值。结论:乙肝表面抗体可通过应用ELISA检测出S/CO值后,能够很容易计算出对应的定量值,适用于未开展乙肝定量检测的实验室应用,有重要临床意义。

Objective To look for the relationship between values of hepatitis B surface antibody S/CO detection and quantitative values. Methods： different concentrations of clinical samples have been brought into the test of hepatitis B by using Time - resolved fluorescence（TRF） immunoassay and enzyme - linked immunosorbent assay （ELISA）. Then draw the scatter plots based on test results, and use SPSS for data analysis. Resuts： S/CO values from ELISA can be converted into quantitative values from TRF with the equation, Y = 26.02 × e0.128x. Conclusion： It is easy to find out Hepatitis B surface antibody levels after having detected S/CO value in application of ELISA. It has important clinical significance to carry out laboratory applications of hepatitis B quantitative detection in this way in the future.