长期使用地西泮对神经活性配体受体相互作用信号通路的影响

Influence of long-term usage of diazepam on neuroactive ligand-receptor interaction signaling pathway

研究长期使用地西泮对小鼠神经活性配体受体相互作用信号通路的影响。通过长期且逐量递增的方式给小鼠连续灌胃地西泮60 d,提取小鼠全脑总mRNA,进行小鼠全基因组芯片检测,运用生物信息学方法筛选出差异表达基因,并通过《京都基因与基因组百科全书》(KEGG)的基因功能通路数据库分析神经活性配体受体相互作用信号通路的变化。结果发现,与正常组比较,地西泮组的神经活性配体受体相互作用信号通路上的16个基因表达发生了显著性变化,其中表达上调的基因有9个,表达下调的有7个。实验结果表明,长期使用地西泮对神经活性配体受体相互作用信号通路具有显著的影响,可能和长期使用地西泮导致机体所产生的不良反应密切相关。

The aim of this work was to study the influence of long term usage of diazepam on neuroactive ligand-receptor interaction pathway. The total mRNA was extracted from the whole brain of mice after long-term usage of diazepam, then hybridized with Oligo microarray and screened by the analysis of gene expression profiles. The differentially expressed genes were screened by bioinformatics and the change of neuroactive ligand-receptor interaction pathway was analyzed through Kyoto Encyclopedia of Genes and Genomes（KEGG）. Compared with the control group, 16 genes transcript level in neuroactive ligand-receptor interaction pathway were significant difference, and 9 genes mRNA expression was up-regulated and 7 genes were down-regulated. The influence of long-term usage of diazepam on neuroactive ligand-receptor interaction signaling pathway was significant, which may be closely related to the adverse reaction after long-term usage of diazepam.